Gymnemagenin benefit for blood sugar control

Gymnemagenin (3 beta,16 beta,21 beta,22 alpha,23,28-hexahydroxy-olean-12-ene), is a sapogenin from Gymnema sylvestre,

Extraction and quantification of gymnemic acids through gymnemagenin from callus cultures of Gymnema sylvestre.
Phytochem Anal. 2006 Nov; Kanetkar PV, Singhal RS, Laddha KS, Kamat MY. Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai, Matungoa, Mumbai 400 019, Maharashtra, India.
The phyto-constituents of Gymnema sylvestre are used in the treatment of diabetes and obesity. The present work reports on the extraction of gymnemic acid through gymnemagenin from callus cultures of G. sylvestre. Components were separated on pre-coated silica gel 60 GF254 plates with chloroform:methanol (8:2) and scanned using a densitometric scanner at 205 nm in the near-UV region. Linearity of determination of gymnemagenin was observed in the range 2-10 microg. The average percentage recovery of gymnemagenin from leaf callus extracts was 99.

Standardisation of Gymnema sylvestre R.Br. by high-performance thin-layer chromatography: an improved method.
Phytochem Anal. 2006 May; Raju VS, Kannababu S, Subbaraju GV. Laila Impex Research Centre, Unit I, Phase III, Jawahar Autonagar, Vijayawada -520 007, India.
An improved high-performance thin-layer chromatographic (HPTLC) method for the standardisation of Gymnema sylvestre is reported. The method involves the initial hydrolysis of gymnemic acids, the active ingredients, to a common aglycone followed by the quantitative estimation of gymnemagenin. The present method rectifies an error found in an HPTLC method reported recently.

Standardisation of Gymnema sylvestre r. Br. with reference to gymnemagenin by high-performance thin-layer chromatography.
Phytochem Anal. 2004 May-Jun; Puratchimani V, Jha S. Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra, Ranchi-835 215, India.
A simple and reproducible HPTLC method for the determination of gymnemagenin in Gymnema sylvestre has been developed. Components were separated on pre-coated silica gel 60 F254 plates with chloroform:methanol (9:1) and scanned using a densitometric scanner in the UV reflectance mode at 290 nm. Linearity of determination of 1 was observed in the range 4-10 microg. The average percentage recovery of 1 from an extract was 99, and the content of 1 in leaves of the title plant was 1.61% (dry weight).